Translational_Unit

Part:BBa_K567015:Design

Designed by: You Wang   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)


PT7-metGN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1437
    Illegal XbaI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1437
    Illegal NheI site found at 1226
    Illegal NotI site found at 1290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1437
    Illegal BamHI site found at 1259
    Illegal XhoI site found at 1211
    Illegal XhoI site found at 1299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1437
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1437
    Illegal XbaI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

overlapping PCR is used


Source

metG from E.coli

References